Immunoprecipitating RISCs from BJAB cells that express BART miRNAs constitutively.
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*Fold enrichment of miRNAs was normalized to the amount of miRNAs in input.Expression levels of cellular miR-150 and EBV’s miRBART10 were measured by stem-loop RT-PCR in BJAB BARTs and BJAB Empty RNAs immunoprecipitated with either anti-Ago2 or anti-GST antibody. Fold enrichment in each experiment was normalized to the Input sample. miR-150 is enriched in α-Ago2 immunoprecipitates in both BJAB BARTs and BJAB Empty cell lines, whereas BART10 is only enriched in immunoprecipitates of BJAB BARTs cells, as anticipated.
创建时间：
2015-12-02
