All the regulated lncRNAs in breast cancer stem cells (BCSCs, CD44+CD24- sorted)MCF-7 cells compared to non-BCSCs (non-CD44+CD24- sorted) MCF-7 cells

Circulating transcriptional landscapes between BCSCs and non-BCSCs were compared using the Affymetrix Human OE LncRNA Microarray with probes for profiling of 63542 human lncRNAs. Goal was to investigate potential lncRNAs involved in BCSCs progression. We used flow cytometry sorting to obtain CD44+CD24- MCF-7 cells and non-CD44+CD24- MCF-7 cells, extracted the total RNAs of these two kinds of cells, and detected the global expression profile of lncRNAs with Affymetrix Human OE LncRNA Microarray.