Cellular and molecular characterization of the dural sinuses as a neuroimmune interface [Myeloid cells]

Meningeal immunity is critical to appropriate CNS function. Here we perform single cell RNA-sequencing to investigate how brain and meningeal stromal populations regulate immune trafficking and retention in young and old mice. At the age of 2 months, WT mice were subjected to one intracisternal injection of Ovalbumin coupled with an Alexa-647 (5ul), and, at the same time, to one intravenous injection of OVA coupled with an Alexa-488. Myeloid cells from the dura were sorted by FACS (CD45+CD11b+ Ly6g-) one hour after both injections. In total, 4 samples (double negative, OVA-488 positive only, OVA 647 positive only, double positive cells) were collected from 20 WT C57BL/6J mice.