Efficient_genome_modification_by_CRISPR_Cas9_nickase_with_minimal_off_target_effects
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/ERP004393
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资源简介：
In order to minimize or eliminate the off-target resulted from the targeting genes in mice by cas9 technology, we have developed a new strategy by using cas9 nickase with paired gRNAs. To study systematically how efficient our strategy is, we would sequence the whole genome of targeted mice produced with our new strategy.
创建时间：
2021-02-04
