Transcriptomic changes in macrophages induced by damage-associated molecular patterns (DAMPs)

Dead cell-derived molecules, typically called as damage-accociated molecular patterns (DAMPs), have been shown to evoke inflammatory responses. Accordingly, it becomes increasingly clear that DAMPs play important role in pathogenesis of multiple diseases including inflammatory diseases and cancer. To get a comprihensive view of DAMP-mediated inflammatory responses, we perfomed microarray analysis on murine macrophages treated by dead cell-derived molecules. Mouse peritoneal macrophages (PECs) were treated by necrotic cell-derived supernatant for 2 hours. Subsequently, PECs were subjected to RNA extraction and hybridization to Clariom S array, Mouse.