H3K27ac ChIP-seq for multiple primary samples, normal tonsil samples and cell lines
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资源简介：
ChIP-seq analysis was performed in primary NKTL (Natural killer T-cell lymphoma) samples, normal tonsil samples and 2 cell lines to analyze acetylation of histone H3K27ac. Three primary NKTL tumor samples and their matched normal tonsils (controls) and NKYS and HANK1 cell lines were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
创建时间：
2023-04-19
