Proteomic analysis of bystander effects induced by irradiated chondrosarcoma cells

To understand the cellular response of bystander cells, we performed a 2D gel-based proteomic study of the chondrocyte receiving the conditioned medium of low dose irradiated chondrosarcoma cells. The proteomic analysis of the bystander chondrocytes highlighted 20 proteins spots that were significantly modified at low dose, implicating several cellular mechanisms, such as oxidative stress responses, cellular motility and exosomes pathways. Several of these candidates and cellular mechanisms were confirmed by functional analysis, such as 8-oxodG quantification, western blot and wound-healing migration tests.