Primers and probe used for bucl amplificationa.

a Primers were designed to generate conserved amplicons within the non-collagenous region of each bucl gene. Primers for bucl6 and bucl15 were designed flanking the collagenous region, which varies in size among strains (S4B Fig). Primers Bucl8_3F/3R were used to generate amplicon from Bp K96243 reference strain, whereas primers Bucl8_2F/2R selectively amplify products from Bp and Bm gDNA and were used in large-scale PCR screening (S4B Fig).b Amplicon sizes expected for the Bp K96243 reference strain.c LNA probe for bucl16 detection; + symbols precede LNA bases.Primers and probe used for bucl amplificationa.