Green spectrofluorimetric assay of dantrolene sodium via reduction method: application to content uniformity testing

The green analysis has turned out to be of a great value in all areas, including pharmaceutical analysis. Thus, it is extremely important to consider the environmental influence of each step in developing any analysis technique.  The present work illustrates a validated, simple and green spectrofluorimetric method for the analysis of dantrolene sodium. The developed process is characterized by being of high sensitivity as well as being relatively inexpensive. The suggested technique based on the formation of a highly fluorescent product of dantrolene sodium via its reduction by the aid of Zn/HCl system. The resulting fluorophore showed a powerful fluorescence at λem 344 nm after excitation at λex 279 nm. Calibration graph revealed a great linear regression (r = 0.9998) within concentration ranging from 0.05 to 2.0 µg mL−1. The suggested method had a very low detection and quantification limit of 0.007 ng mL−1 and 0.019 ng mL−1, respectively. The applied technique was effectively used in...