Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein.
收藏PubMed Central1995-04-11 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC42119/
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资源简介：
The SSN6-TUP1 protein complex represses transcription of diversely regulated genes in the yeast Saccharomyces cerevisiae. Here we present evidence that MIG1, a zinc-finger protein in the EGR1/Zif268 family, recruits SSN6-TUP1 to glucose-repressed promoters. DNA-bound LexA-MIG1 represses transcription of a target gene in glucose-grown cells, and repression requires SSN6 and TUP1. We also show that MIG1 and SSN6 fusion proteins interact in the two-hybrid system. Unexpectedly, we found that LexA-MIG1 activates transcription strongly in an ssn6 mutant and weakly in a tup1 mutant. Finally, LexA-MIG1 does not repress transcription in glucose-deprived cells, and MIG1 is differentially phosphorylated in response to glucose availability. We suggest a role for phosphorylation in regulating repression. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1995-04-11
