Dissecting the multi-omic atlas of the exosomes released by human lung adenocarcinoma stem-like cells

Lung adenocarcinoma is heterogeneous and hierarchally organized, with a sub-population of stem-like cells (CSC) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. In order to characterize the repertoire of exosomal RNAs, we performed Ribo-zero RNA sequencing, which can capture both polyA and non-polyA RNAs, on lung adenocarcinoma stem-like cells and lung adenocarcinoma bulk cells as well as their derived exosomes, with two replicates for each.