#GliaMorph - Double-transgenic Müller Glia Data at 3dpf and 5dpf Zebrafish

Images of zebrafish retina at 3dpf and 5dpf in the double-transgenic Tg(TP1bglob:VenusPest)s940 (Ninov et al., 2012) and Tg(CSL:mCherry)jh11 (also known as Tg(Tp1bglob:hmgb1-mCherry)jh11) (Parsons et al., 2009). GliaMorph toolkit to quantify glia shape in 3D: https://github.com/ElisabethKugler/GliaMorph <strong>step1_cziToTiffTool.ijm</strong>: Macro for czi to tiff conversion <strong>step2_DeconvolutionTool.ijm</strong>: Macro for deconvolution of confocal images using theoretical or experimental PSF <strong>step3_90DegreeRotationTool.ijm</strong>: Macro for 90 degree rotation - optional before rotationTool <strong>step4_subregionTool.ijm</strong>: Semi-automatic ROI selection tool with ROI selection from MIP <strong>step4_subregionToolWithinStack.ijm</strong>: Semi-automatic ROI selection tool within stack with ROI within stack <strong>step5_splitChannelsTool.ijm</strong>: Macro to split channels and save them in separate folders <strong>step6_zonationTool.ijm</strong>: Analysis of zebrafish retina zonation based on intensity profiles <strong>step7_SegmentationTool.ijm</strong>: Segmentation of MG cells <strong>step8_QuantificationTool.ijm</strong>: Quantification of MG features in segmented images