CRAC of yeast RNA polymerase II in the absence or upon depletion of Xrn1

We report RNA polymerase II occupancy profiles across the genome of S.cerevisiae strains deleted or depleted for the protein Xrn1. This allowed investigating the role of Xrn1 in RNA polymerase II transcription. Overall design: Xrn1 was depleted from the cell using the auxin-degron method. Nuclear depletion was also studied, using the anchor away methodology. In both cases several time points of depletion have been analyzed, including no addition of auxin (auxin-degron method) or rapamycin (anchor-away technique). An xrn1? strain was also studied. The nascent RNA was crosslinked to RNAPII and sequenced after purification of the HTP-tagged large subunit Rpb1 under stringent and denaturing conditions. This reveals the position of the polymerase at the nucleotide resolution level.