Sequencing data of primary lung tumors and metastatic liver tumors in RP mice with SCLC

In RP (Rb1fl/fl;Trp53fl/fl) mice, SCLC phenotype tumors were established eight months after intratracheal injection of an adenovirus carrying Cre recombinase, driven by the CMV promoter. Primary lung tumors and metastatic liver tumors from the same mouse were collected for sequencing, resulting in a dataset.A similarity analysis was conducted between this dataset and the subpopulations with high and low autophagic flux in our RP?G (Rb1fl/fl;Trp53fl/fl;GFP-LC3-RFP-LC3?G) mice. The analysis revealed that the subpopulation with high autophagic flux exhibited greater similarity to the metastatic data. Overall design: In RP (Rb1fl/fl;Trp53fl/fl) mice, SCLC phenotype tumors developed within eight months following intratracheal injection of an adenovirus carrying Cre recombinase, driven by the CMV promoter. Primary lung tumors were formed in the lungs, and metastatic tumors were observed in the liver. We collected two lung tumor nodules and one liver metastatic tumor nodule from the same mouse for sequencing analysis.