Additional file 8: of Do novel genes drive morphological novelty? An investigation of the nematosomes in the sea anemone Nematostella vectensis

Summary of everything. Sheet 1: Stress Gene Survey. List of tissue-specific targets and their reciprocal best blast hit (accession ID) from the dataset presented by Reitzel et al [63]. Included fields: Tissue, unique transcript ID (NvecRef ID), reciprocal best blastx hit (Hit), stressor type (pathogen, wound, or chemical). Transcripts with reciprocal best blast hits involved in both pathogen and wound stress are indicated as: Pathogen.Wound. Sheet 2: Transcriptome Summary. Summary of all 17,313 transcripts used for differential expression and orthology analyses. Each entry contains the following: unique transcriptome ID (NvecRef ID), transcript length (in nucleotides), orthology group ID (GrID): N – Nematostella-specific, E – Edwarsiidae-specific, A – anthozoan-specific, C – cnidarian-specific, M – conserved/metazoan, number of tissues (out of three) in which the transcript is expressed, differential expression result (UP/mes is upregulated in the mesenteries relative to both other tissues), mean counts across all tissues (AllMean) and across replicates within a tissue (MesMean, NemMean, TenMean), log2 fold change for all pairwise tissue comparisons (negative values indicate upregulation in the second tissue), adjusted p-values/false discovery rates for all pairwise comparisons, and raw counts for each tissue replicate. Sheet 3: Alien Index Results. Fields: unique transcriptome ID (NvecRef ID), alien index (AI), % identity and coverage of the top blast hit, and whether the sequence was deemed present in the genome (http://genome.jgi.doe.gov/Nemve1/Nemve1.home.html). Sheet 4: Primers. Sequences for all primers used in qPCR and standard PCR analyses. Primer sequences for each transcript are presented 5’ → 3’. (XLSX 4037 kb)