five

Bridgin connects the outer kinetochore to centromeric chromatin

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NIAID Data Ecosystem2026-03-12 收录
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https://www.omicsdi.org/dataset/jpost/PXD021072
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In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pro were processed with MASCOT via Proteome discoverer2.2. Validation of peptide and protein identifications were performed with Scaffold proteome software.
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2020-09-23
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