Data_Sheet_2_Expansion and Divergence of Argonaute Genes in the Oomycete Genus Phytophthora.FASTA

Modulation of gene expression through RNA interference is well conserved in eukaryotes and is involved in many cellular processes. In the oomycete Phytophthora, research on the small RNA machinery and function has started to reveal potential roles in the pathogen, but much is still unknown. We examined Argonaute (AGO) homologs within oomycete genome sequences, especially among Phytophthora species, to gain a clearer understanding of the evolution of this well-conserved protein family. We identified AGO homologs across many representative oomycete and stramenopile species, and annotated representative homologs in P. sojae. Furthermore, we demonstrate variable transcript levels of all identified AGO homologs in comparison to previously identified Dicer-like (DCL) and RNA-dependent RNA polymerase (RDR) homologs. Our phylogenetic analysis further refines the relationship of the AGO homologs in oomycetes and identifies a conserved tandem duplication of AGO homologs in a subset of Phytophthora species.

通过RNA干扰调控基因表达在真核生物中得以高度保守，并参与众多细胞过程。在卵菌门植物病原体疫霉菌中，对小RNA机制及其功能的研究已开始揭示其在病原体中的潜在作用，但仍有诸多未知。我们考察了卵菌基因组序列中的Argonaute（AGO）同源物，尤其是在疫霉菌属中，以期对这一高度保守的蛋白家族的进化获得更为清晰的认知。我们鉴定了多个代表性卵菌和丝状菌门物种中的AGO同源物，并在P. sojae中注释了代表性同源物。此外，我们比较了所鉴定所有AGO同源物的转录水平与先前鉴定的Dicer样（DCL）和RNA依赖性RNA聚合酶（RDR）同源物。我们的系统发育分析进一步细化了卵菌中AGO同源物之间的关系，并识别出疫霉菌属中部分物种中AGO同源物的保守串联重复。