Genetic ablation of maternal SoxB1 results in the delay of gastrulation gene regulatory program in zebrafish

Purpose: Find out the consequences of Sox19b binding to the nucleosome distribution after ZGA Methods: MNase-seq of WT and MZsox19b mutant zebrafish embryos at dome stage, building up nucleosome occupancy profiles Results:Maternal Sox19b is involved in the nucleosome displacement from HNARs at the zygotic genome activation. Conclusions: Nucleosome clearance by maternal Sox19b is essential for proper onset and timing of gastrulation. Overall design: Nucleosome occupancy profiles of dome staged wild type (WT) and MZsox19b zebrafish mutant were generated by MNase treatment followed by deep sequencing, using Illumina HiSeq 2500.