Transinfection of Wolbachia wAlbB into Culex quinquefasciatus mosquitoes does not alter vector competence for Hawaiian avian malaria (Plasmodium relictum GRW4)

Avian malaria is expanding upslope with warmer temperatures and driving multiple species of Hawaiian birds towards extinction. Methods to reduce malaria transmission are urgently needed to prevent further declines. Releasing Wolbachia-infected incompatible male mosquitoes could suppress mosquito populations and releasing Wolbachia-infected female mosquitoes (or both sexes) could reduce pathogen transmission if the Wolbachia strain reduced vector competence. We cleared Culex quinquefasciatus of their natural Wolbachia pipientis wPip infection and transinfected them with Wolbachia wAlbB isolated from Aedes albopictus. We show that wAlbB infection was transmitted transovarially, and demonstrate cytoplasmic incompatibility with wild-type mosquitoes infected with wPip from Oahu and Maui, Hawaii. We measured vector competence for avian malaria, Plasmodium relictum, lineage GRW4, of seven mosquito lines (two with wAlbB; three with natural wPip infection, and two cleared of Wolbachia infection)..., This dataset comes from a series of experiments with mosquitoes, wolbachia, and avian malaria., , # Transinfection of Wolbachia wAlbB into Culex quinquefasciatus mosquitoes does not alter vector competence for Hawaiian avian malaria (Plasmodium relictum GRW4)

[https://doi.org/10.5061/dryad.k6djh9wf4](https://doi.org/10.5061/dryad.k6djh9wf4)

This dataset includes R code and data for:

* Vector competence experiments
* Salivary gland malaria DNA comparison
* Mosquito Feeding success
* Cytoplasmic incompatibility/egg viability
* Mosquito Fecundity
* Mosquito Survival
* Avian malaria infection in canaries
* Inheritance of Wolbachia

## Description of the data and file structure

**In all .csv files empty cells indicate no data.**

**Data files and column descriptions:**

Note that when .csv files are loaded into R, blank spaces in column names are replaced with a \".\", so \"Male Strain\" becomes \"Male.Strain\"

### **2023 Verily Bloodfed Mosquito Sample Tracker -** **Bloodfed Mosquito Tracker 12\_29\_23.csv**

The column names and explanation are:

\[1] \"UNQ_ID\" - ID for...