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MFGE8 induces anti-PD-1-therapy resistance by promoting extracellular vesicle sorting of PD-L1

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NIAID Data Ecosystem2026-05-02 收录
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To identify which E3 ligases ubiquitinate PD-L1, we overexpressed PD-L1-Flag in HEK293 cells and used anti-Flag magnetic beads to pull down PD-L1 from cell lysates. The interacting proteins were then analyzed by mass spectrometry, revealing that three E3 ligases interact with PD-L1.

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2025-05-07
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