Adipose depot differences in high fat fed mice

Purpose : To understand differences in microRNA (miRNA) signatures of different adipose tissue depots to gain mechanistic insight regarding their contribution to metabolic disorders in obesity. Method : We performed small RNA-sequencing of brown, subcutaneous and visceral adipose depots from high fat diet-fed mice (mice were fed a 45% kcal fat diets from 5-6 weeks of age for 11 weeks). The main animal study reference: Kalupahana et al., J. Nutr, 2010 Results:Using the Gunaratne Next Generation pipeline (published in Creighton et al. 2009) miRNA expression profiles were identified. Counts of each unique read were normalized to total usable reads, and had 40 counts added. We mapped about 13.8 million sequence reads per sample to the Mus musculus genome (build mm 10). Altogether 1251 miRNAs were identified in three adipose tissues and out of which 246 showed differential expression with 1.25 or more fold change and p value <0.05 in one or more of the pairwise comparisons of the 3 adipose depots. Hierarchical clustering, demonstrated that biological replicates behaved appropriately as they were clustered together. BAT exhibited a different gene/ miRNA expression pattern than the 2 other WATs (SAT and VAT). SAT and VAT showed a closer relationship with each other than with BAT Conclusion : High fat diet differentially regulate specific miRNAs expression in different adipose tissue depots Overall design: MicroRNA profiles of brown, subcutaneous and visceral adipose tissues of 16-17 wk old wild type high fat diet induced obese C57L/6 mice were generated by small RNA sequencing, in triplicate, using Illumina Genome Analyzer II.