Single cell transcriptomics of cardiac immune cells after pressure overload

Cardiac immune cells play important roles in orchestrating the response of the heart to stress and injury. The precise role of immune cells in the early phase after cardiac pressure overload is unclear. Clustering analysis of >33,000 sequenced single cells identified 25 immune cell populations including 12 macrophage and monocyte populations. Overall design: Mice were subjected to sham or TAC surgeries, followed by cardiac non-cardiomyocyte isolation 1 week later. Immune cells were enriched by magnetic selection, followed by barcoded antibody incubation to allow identification of 4 sham and 6 TAC samples after 10x Genomics droplet sequencing.