CIL:40249, Canis lupus familiaris, epithelial cell. In Cell Image Library
收藏DataCite Commons2025-10-31 更新2026-05-06 收录
下载链接：
https://library.ucsd.edu/dc/object/bb8267333g
下载链接
链接失效反馈官方服务：
资源简介：
MDCK cells stably expressing two internal 4C domains in the C-terminus (FLNCCPGCCME)-tagged Cx43 (Cx43-4C309/337) were labeled for 1 h at 37 degrees C with 180 nM FlAsH-EDT2/12.5 uM EDT in Hanks balanced salt saline (HBSS). Free and non-specifically bound FlAsH was removed by washing with 2,3-dimercapto-1-propanol (BAL, 500 uM, 20min at 37 degrees C in HBSS). Time-lapse imaging was conducted using an Olympus FluoView1000 confocal microscope equipped with a temperature controlled chamber (at 37 degrees C) and a 60X 1.42 NA objective. Medium: Opti-MEM supplemented with 5% FBS covered with a glass coverslip.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-06-17
