Experimental autoimmune encephalomyelitis (EAE) model in Nfkbiz−/− mice.

(A) Disease progression of EAE in Nfkbiz+/+ (n = 11–13) and Nfkbiz−/− mice (n = 9–11). (B–D) Analysis of mice 12 days after immunization. (B) Cytokine profile of CD4+ cells in draining LNs. (C, D) Measurement of IL-17A (C) and IFN-γ (D) supernatant concentrations by ELISAs (Nfkbiz+/+: n = 5; Nfkbiz−/−: n = 6), using cultured draining LNs incubated in the presence or absence of MOG peptide (10 ng/ml) for 72 h. Data shown represent mean ± S.E. Paired data were evaluated using the Student's t test. *ppNfkbiz+/+ and Nfkbiz−/− mice were sacrificed and their lumber section of spinal codes were collected. Three-micrometer-thick sections were stained with hematoxylin and eosin (HE), Klüver-Barrera staining (KB) or galectin-3 (Gal-3) immunohistochemistry. Serial sections were used for HE staining, KB staining and Gal-3 immunohistochemistry. Arrowheads in HE staining and KB staining indicate the demyelinated lesions. Data are representative of 3 independent experiments.