Transcriptome-wide impact of progesterone in the medio-basal hypothalamus of the ewe

Here we used RNAseq to assess the impact of progesterone (P4) on the transcriptome of the medio-basal hypothalamus of ewes. Ewes were treated with a vaginal fluogestone acetate sponge in mid-February. The sponge was removed in early March and ewes received a PMSG injection. Animals were then killed either 2 days (follicular phase) or 12 days (luteal phase) later. Overall design: The study was performed in adult ewes of the Ile-de-France breed (hereafter IF). The experiment included 20 ewes : 10 in Follicular phase and 10 in Luteal phase. RNA was extracted from each ewe MBH. Then, for each condition 5 random equimolar mixes of mRNA (n=2 per mix) were used for RNAseq --> 5 biological replicates.