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qRT-PCR data and PCR efficiency of the different ptimers used.
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创建时间：
2012-01-01
相关数据集
TaqMan primers/probes targeting specific mRNAs used for mRNA quantification.
TaqMan primers/probes targeting specific mRNAs used for mRNA quantification.
Figshare2025-11-07 更新40
Primer sequences used for mRNA quantification by real-time PCR.
11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; GR, glucocorticoid receptor; C/EBPα, CCAAT/enhancer-binding protein α; C/EBPβ, CCAAT/enhancer-binding p
Figshare2015-12-02 更新10
Next Generation Sequencing analysis of VL and VH regions following PCR #2 using standard or rh-PCR primers.
Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta
NIAID Data Ecosystem30
Primers and probes for quantitation of mRNA by Q-PCR for rabbit BAFF, BR3, PPIA control and β2 microglobulin (B2M).
aThe total volume of PCR reaction was 25 µl and the PCR conditions were as follows: 50°C for 2 min, 95°C for 10 min, followed by 40 cycles of 95°C, 15 s for denaturation and 60°C, 1 min for annealing
NIAID Data Ecosystem20
