Transcriptome analysis of mouse follicular (FO) and marginal zone (MZ) B cells from control mice and of MZ B cells from mice overexpressing AKT-1 in B cells

The purpose of this experiment is to delineate whether Akt over-expression is sufficient to induce a marginal zone-like phenotype in B cells at the molecular level. Mice expressing constitutively Akt in B cells have almost only marginal zone B cells in secondary lymphoid organs, as much as we can say through characterization of cell surface receptor by flow cytometry. These analyses were conducted in order to gain a more precise understanding of the relationship between WT marginal zone B cells and marginal zone B cells produced as a result of constitutive Akt signalling. The experiments include a total of 9 arrays, each corresponding to a distinct biological sample. There are 3 arrays (3 samples) for FO B cells from control mice (Cd19-cre mice; Rickert et al. 1997), 3 arrays for MZ B cells from the same control mice, and 3 arrays from mice expressing an hyperactive AKT-1 in B cells (AKTboe mice that are Cd19-cre x ROSA26-LoxP-STOP-LoxP-AKT1-ires eGFP; see PMID: 33140819 for the description of this allele)