Size-dependent behavioral and antennal responses to doses of (+)-isopinocamphone and 1,8-cineole mixed with pheromone: a potential host selection strategy in female Ips typographus L.

Context:Ips typographus, a major pest of Norway spruce (Picea abies) in Europe, is experiencing more frequent outbreaks due to climate change. These outbreaks involve shifts in population dynamics and phenotypic traits, influencing beetle responses to olfactory cues from stressed host trees.
Aims:The study examines the size-dependent behavioral and antennal responses of female I. typographus to two host selection–deciding volatiles with contrasting ecological roles: 1,8-cineole, which inhibits attraction to unsuitable trees, and (+)-isopinocamphone, a pheromone synergist. Size-linked morphological and olfactory adaptations may influence females' ability to select suitable host trees for reproduction.
Methods:In field trap experiments conducted in 2019 and 2022, the body size of I. typographus females caught in response to different doses of (+)-isopinocamphone or 1,8-cineole in combination with pheromone was compared. Female Ips typographus were sorted based on body length, the size of ..., 1) Study Location and years:
The experimental areas: Norway spruce forest, the natural habitat of Ips typographus, at 600 m a.s.l. within the Forest CZU property, Kostelec nad Černými lesy, Czech Republic.

2019 site: Coordinates 49°56′02″N, 14°52′21″E.
2022 site: Coordinates 49°55′57″N, 14°55′13″E.

2) Time Frame and field experiments:

       Experiments ran from June 3 to July 28 in both 2019 and 2022.

2.1 Trap Type:

Cross-vane Ecotraps (Fytofarm, Slovak Republic) were used for the experiments.

2.2 Trap Placement:

Traps were positioned 15 meters apart, 1.5 meters above the ground, and approximately 30 meters from the forest edge.

2.3 Experimental Design (2019):
Seven traps were used.

Six traps were baited with a pheromone and three different doses (low, medium, high) of either 1,8-cineole or (+)-isopinocamphone.
One control trap was baited with pheromone alone.

2.4  Experimental Design (2022):
Two experimental blocks were established:

One block t..., # Data from: Size-dependent behavioral and antennal responses to doses of (+)-isopinocamphone and 1,8-cineole mixed with pheromone: a potential host selection strategy in female Ips typographus L.

Dataset DOI: [10.5061/dryad.rxwdbrvn1](10.5061/dryad.rxwdbrvn1)

## Description of the data and file structure

Size-dependent behavioral and antennal responses to doses of (+)-isopinocamphone and 1,8-cineole mixed with pheromone: a potential host selection strategy in female *Ips typographus* L.

#### **Description of the data and file structure**

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###### Trapping experiments: Plot, traps and tested area

The trapping experiments were conducted in 2019 and 2022 at the Forest CZU property in Kostelec nad Černými lesy, Czech Republic. The experiments took place in a mature, 100-year-old Norway spruce forest, a natural habitat for *I. typographus*, located at 600 m above sea level. In 2019, the experiment was conducted at coordinates (49°56′02″N, 1...,