Locomotor behavior summary for <i>dot-Gal4</i> mediated RNAi knockdown of PDF.

Loss of abdominal PDF expression by Dot-Gal4-driven uas-PDFrnai and uas-Dcr2 did not result in a decrease in rhythmicity under DD. ANOVA revealed a significant difference between genotypes in τ, but a Tukey's test did not detect differences between y w,uas-Dcr2;Dot-Gal4/+;uas-PDFrnai/+ flies and controls (p>0.05 for each pair-wise comparison). ANOVA revealed a difference between genotypes in the power of free-running rhythms (ANOVA p<0.0001) and in evening peak phase under LD (ANOVA p<0.0001), however these differences reflected the differences observed between y w,uas-Dcr2;Dot-Gal4/+ and y w,uas-Dcr2/y;;uas-PDFrnai/+ and were not correlated with PDF levels in the abdominal PDF neurons.