Transcriptomic profile of IEC4.1 cells treated with an ASO to lncRNA XR_001779380 in response to IFN-gamma

LncRNAs may play an important role in intestinal epithelial cell autonomous defense against microbial infection. Our previous study showed that lncRNA XR_001779380 can interact with Prdm1 to modulate gene transcriuption in intestinal epithelial cells and may regualte IFN-gamma-stimulated gene transcription. Here we designed an antisense oligos-morpholine (ASO4) targeting the sequence of lncRNA XR_001779380 to interfere with its interaction with PRDM1. Murine intestinal epithelial cells (IEC4.1 cells) were treated with ASO4 or a standard control ASO-morpholine (ST) for 24h, followed by exposure to IFN-gamma (1 ng/ml) for 4 hours. Then total RNA was collected for sequencing via DNBSEQ platform to obtain a comprehensive view of the transcriptome. Overall design: Mouse intestine epithelial IEC4.1 cells were tansfected with the ST or ASO4 for 24h. The transfected cells were treated with or without IFN? for 4 hours. The RNA expression profile of these cells were compared.