Joint-Structural Biology Group (JSBG) BAG proposal for ID29 beamline

The aim of the JSBG BAG proposal for ID29 beamline is to develop new methods for time-resolved serial crystallography experiments at 4th generation source and to study time dependent structural conformation of biological macromolecules that are studies at the ESRF-EMBL-GR Joint Structural Biology Group. Method developments include the study of radiation damage at high flux densities and with partially polychromatic beam (1% and 0.3%), the implementation and development of various sample delivery methods - high viscosity, GDVN, mix-inject injectors, microfluidics and fixed targets and the integration of software for large amount of SSX data reduction, data-acquisition software for JungFrau 4M detector, and various data collection strategies for SSX experiments. Biological systems include the study of photoactivatable proteins, dynamics/inhibitor mechanisms of non-photoactivatable proteins by using photo-cage compounds and enzymatic systems.