Identification of slow-cycling cells in Ewing sarcoma
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE204671
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We identified slow-cycling cells (SCCs) in Ewing sarcoma using a label retention assay with CFSE. We labeled cells of SK-ES-1, an Ewing sarcoma cell line, with CFSE. After 5 days culture, we isolated cells retaining strong fluorescence (upper, ~10%) as SCCs and other cells (lower, ~90%) as non-slow-cycling cells (non-SCCs) using FACS AriaTM Ⅲ cell sorter. Comparative gene expression profiling analysis of RNA-seq data for SCCs and non-SCCs.
创建时间：
2022-10-18
