B cell single cell seq in allergen-specfic immunotherapy

IgE-producing memory cells are hard to detect in the periphery. Here, we sorted IgE+ memory B cells from allergic patients who had received allergen-immunotherapy for grass pollen. Cells were FACS-sorted and subseuqenlty sequenced by single cell RNAseq to determine isotype and phenotype. Two out of 90 cells were determiend to be true IgE producing memory B cells. Overall design: 95 single B cells were sorted, no replicates were perfromed. One control sample was included.