Supplementary Table 1.xlsx

Amplification of <i>MYCN</i> is observed in high-risk neuroblastomas (NBs) and is associated with a poor prognosis. <i>MYCN</i> expression is directly regulated by multiple transcription factors, including OCT4, MYCN, CTCF, and p53 in NB<i>.</i> Our previous study showed that inhibition of p53 binding at the <i>MYCN</i> locus induces NB cell death. However, it remains unclear whether inhibition of alternative transcription factor induces NB cell death. In this study, we revealed that the inhibition of OCT4 binding at the <i>MYCN</i> locus, a critical site for the human-specific OCT4–MYCN positive feedback loop, induces caspase-2-mediated cell death in <i>MYCN</i>-amplified NB. We used the CRISPR/deactivated Cas9 (dCas9) technology to specifically inhibit transcription factors from binding to the <i>MYCN</i> locus in the <i>MYCN</i>-amplified NB cell lines CHP134 and IMR32. After the inhibition of OCT4 binding at the <i>MYCN</i> locus in CHP134 and IMR32, we performed long-read and short-read RNA sequencing analysis. This dataset includes gene information, including open reading frame (ORF) dominance score and normalized expression counts of each transcript (n=3 per condition).