Ancistrocladinium A modulates RNA splicing, cellular stress response and induces cell death in proteasome inhibi-tor-resistant multiple myeloma cells: A promising therapeutic agent candidate

Analysis of gene expression alterations and RNA splicing events after Ancistrocladinium A treatment of INA-6 multiple myeloma cell line Ancistrocladinium A inan N,C-coupled naphthylisoquinoline alkaloid was analyzed for its ability to act as efficient anti-myeloma agent. The mode of action was unknown. We analyzed the effect of Ancistrocladinium A on the multiple myeloma cell line INA-6 on RNA level and identivied differentially regulated genes on the one hand and splicing events on the other hand gaining a first insight into the mode of action of Ancistrocladinium A. INA-6 cells were treated with PBS as solvent control or 3 µM Ancistrocladinium A followed by RNA extration and analysis using the Clariom D human array. Experiment was performed in quadruplicates. Analysis to determine differences between PBS treated cells and Ancistrocladinium A treated cells was perfomed using the Transcriptome Analysis Console Software 4.0 (TAC4.0, Affymetrix).