Lee2010_ThrombinActivation_OneForm_minimal

Chang Jun Lee, Sangwook Wu, Changsun Eun & Lee G. Pedersen. A revisit to the one form kinetic model of prothrombinase. Biophysical Chemistry 149, 1-2 (2010). Thrombin is generated enzymatically from prothrombin by two pathways with the intermediates of meizothrombin and prethrombin-2. Experimental concentration profiles from two independent groups for these two pathways have been re-analyzed. By rationally combining the independent data sets, a simple mechanism can be established and rate constants determined. A structural model is consistent with the data-derived finding that mechanisms that feature channeling or ratcheting are not necessary to describe thrombin production.