Single-cell RNA-Seq of human primary lung and bronchial epithelium cells

This dataset contains count matrices and per-cells metadata tables for RNA sequencing of 39778 single nuclei from healthy primary lung samples of 12 lung adenocarcinoma patients as well as 17451 single human bronchiole epithelial cells from 4 donors. All samples were processed using the 10X Genomics Chromium platform with v2 chemistry and sequenced with one sample per lane on an Illumina HiSeq4000. Reads were aligned to the hg19 reference genome version 1.2.0 obtained from 10X Genomics. Data processing was performed using Seurat3. The metadata table includes patient ID, sex, age, smoking status, and cell type, as well as QC statistics (number of genes, number of cells, ratio of mitochondrial reads).

本数据集汇集了来自12例肺腺癌患者健康原发肺样本的39778个单核RNA测序的计数矩阵以及每个细胞的元数据表，以及来自4位捐赠者的17451个单个人支气管上皮细胞的元数据表。所有样本均采用10X Genomics Chromium平台v2化学制剂进行处理，并在Illumina HiSeq4000上进行单样本每通道测序。测序读段被比对至由10X Genomics提供的hg19参考基因组版本1.2.0。数据处理采用Seurat3软件完成。元数据表包含患者ID、性别、年龄、吸烟状况、细胞类型以及质量控统计信息（基因数量、细胞数量、线粒体读段比率）。