The loci of active HERV-Hs in colon tumor and adjacent normal tissues.

*Active elements individually contributing to more than 10% of the transcripts in tumor or adjacent normal samples are highlighted in boldface and their transcript abundances are indicated in the ‘Abundance’ column (individual and total, respectively).
**HERV-HX is the colon cancer-related HERV-H element identified by us previously [14]. Inserts of PCR product clones were all HERV-HX fragments but not spliced sequences, in concordance with our previous finding that no spliced transcripts were produced from HERV-HX in colon tumor samples.
***The element located at 1q31.3 consists of 5′LTR and 3′LTR, with the entire protein coding region (gag-pro-pol-env) missing.
****Open reading frames (ORFs) were predicted by the online program ORF Finder and putative peptide sequences were subjected to Blastp search against the Non-redundant protein sequences at http://www.ncbi.nlm.nih.gov/. Only predicted ORFs≥303 nt (peptide sequence ≥100 aa) and with Blastp matches are included. RT, Reverse transcriptase; CREB5, cAMP response element-binding protein 5; Gag, group-specific antigen.