Figure 2D - EPR10411 + 11A9 Raw Image Files

Raw image files to:
Figure 2. Further analysis of Ago2 localization to the iRBC. (A)
Binding sites of aAgo2 antibodies used. The monoclonal antibodies 11A9
and EPR10411 were raised against an N-terminal and a centrally located
peptide epitope, respectively. The antibody 2E12-1C9 is a monoclonal
antibody raised against the whole C-terminus of hAgo2. (B) IFA of
uninfected RBCs. Yellow: Ago2 (EPR10411), magenta: Ago2 (either 2E12-1C9
(top) or 11A9 (bottom)). (C) IFA of P. falciparum 2004 parasites at 10,
22, 34 and 46 hours post invasion (hpi) and mature PfNF54
gametocytes. Yellow: Ago2 (EPR10411), magenta: Ago2 (2E12-1C9). Contrast
and brightness of EPR10411 panels were individually adjusted to
optimise image quality, thus, signal strength cannot be compared between
panels. (D) IFA of P. falciparum 2004 parasites at 10 and 46 hpi.
Yellow: Ago2 (EPR10411), magenta: Ago2 (11A9). Nuclei were stained with
Hoechst (blue). Images were taken on a Leica widefield microscope (60x
objective). Representative images of at least 5 per condition are shown.
Controls using only a secondary antibody revealed no unspecific
staining. Scale bar indicates 5 µm.