RNA immunoprecipitation sequencing (RIP-seq) in four types of cancer cell lines. RNA immunoprecipitation sequencing (RIP-seq) in four types of cancer cell lines

The ribosomal protein S6 (RPS6) antibody used for RIP to measure the landscape associated RNA by sequencing Overall design: RNA immunoprecipitation (RIP) was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer’s instructions. An aliquot of lysate was removed as an input control.RNA sequencing was performed with Illummina HiSeq X Ten Sequencer