The comparison of pKa values and n coefficients obtained from the carboxy-SNARF-1 calibration in the mitochondrial matrix by fitting of spectral intensities at various wavelengths and intensity ratios to various equations described in the text.

Data for three independent determinations (data sets 1, 2 and 3) are given explicitly. Values marked with italics have significantly larger errors compared to other calculation methods using explicit n value fitting.