Supporting data for "Chromosome-level assembly of the common vetch reference genome (Vicia sativa)"

<i>Vicia sativa L. </i> (Common Vetch, n= 6) is an annual, herbaceous, climbing legume that is distributed in tropical, sub-tropical and temperate climates. Originating in the Fertile Crescent of the Middle East, <i>V. sativa</i> is now widespread and grows in the Mediterranean basin, West, Central and Eastern Asia, North and South America. <i>V. sativa</i> is of economic importance as a forage legume in countries such as Australia, China, and the USA and contributes valuable nitrogen to agricultural rotation cropping systems. To accelerate precision genome breeding and genomics-based selection of this legume, we here present a chromosome-level reference genome sequence for <i>V. sativa. </i> <br>We applied a combination of long-read Oxford Nanopore sequencing, short-read Illumina sequencing, and high-throughput chromosome conformation data (CHiCAGO and Hi-C) analysis to construct a chromosome-level genome of <i>V. sativa</i>. The chromosome-level assembly of six pseudo-chromosomes has a total genome length of 1.9 gigabases (Gb) with a median contig length of 684 kb. Benchmarking Universal Single-Copy Orthologs (BUSCO) of the assembly demonstrated a very high completeness of 98% of the dicotyledonous orthologs. RNA-seq analysis and gene modeling enabled the annotation of 58,415 protein-coding genes. <br>The high-quality chromosome-level genome assembly of <i>V. sativa</i> will provide novel insights into vetch genome evolution and be a valuable resource for genomic breeding, genetic diversity and for understanding adaption to diverse arid environments