TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness

We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.

本研究构建了3种植物病原菌Ralstonia分离株的转座子插入文库，采用pKMW3，一种随机条形码标记的Mariner转座子载体。在R. pseudosolanacearum GMI1000、R. solanacearum IBSBF1503和R. syzygii PSI07中创建了突变文库。（注：一些菌株的基因组仍被归类为R. solanacearum，但此处所列名称反映了当前的分类学）。关于质粒参考信息，请参见PMID: 25968644，以及TnSeq/BarSeq实验方案。