Carbosilane Dendrimer 2G-NN16 Represses Tc17 Differentiation in Primary T CD8+ Lymphocytes

We studied changes in gene expression induced by the
carbosilane
dendrimer 2G-NN16 to evaluate their potential as a vehicle for gene
therapy and as medication. Global gene expression profiles on CD8+
T lymphocytes reveal that ribosomal proteins are induced in the presence
of 2G-NN16. IL17A and IL17F, the principal interleukins secreted by
Tc17 cells, a subset of CD8+ T lymphocytes, were down-regulated when
cultured in the presence of this dendrimer. Microarray results were
confirmed by real time quantitative reverse transcriptase polymerase
chain reaction (qRT-PCR). 2G-NN16 also showed a high potential for
in vitro inhibition of Tc17 differentiation of CD8+ T lymphocytes
in the presence of the Tc17 differentiation molecules IL6 and TGF-B1.
These findings suggest that 2G-NN16 could facilitate drug delivery
and may be used to treat inflammatory processes driven by Tc17 cells.