PRO-seq assay in adult ventricular cardiomyocytes. PRO-seq assay in adult ventricular cardiomyocytes

PRO-seq assay was performed for the quantitative detection of nascent pre-mRNA transcripts induced by phenylephrine (PE) and adenoviral overexpression of SRF serine-103 mutants in adult ventricular cardiomyocytes Overall design: Adult ventricular cardiomyocytes were stimulated with PE 24 hours after adenoviral infection with SRF WT, S103A and S103D mutants. Nuclei were collected 1 hour after PE treatment, then subjected to precision nuclear run-on sequencing (PRO-seq).