β-galactosidase reporter assays for consensus Correia ends.
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https://figshare.com/articles/dataset/_946_galactosidase_reporter_assays_for_consensus_Correia_ends_/475637
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1The consensus CE ends shown in Figure 1B (along with 4 nucleotides immediately upstream of the Correia repeat and the downstream flanking dinucleotide “AT”) were cloned into a low-copy lacZ reporter plasmid (pRC746). Details are provided in Table S1. The E. coli host strain is MC4100.
2β-galactosidase activity is measured in Miller units. The following formula was used to calculate Miller units: [(spectrophotometer reading at 420 nm wavelength)×1000]/[(spectrophotometer reading at 600 nm wavelength)×(cell volume (mls))×(time (mins))].
3Based on results from 3 independent experiments.
4Relative to vector control (pRC746).
5Unless designated otherwise, the base at position 128 is cytosine.
6Altered −10 and −35 box sequences are shown in Figure 1B.
创建时间：
2011-01-20
