Comparison of transcriptome in gonads and somatic tissues in mice. Comparison of transcriptome in gonads and somatic tissues in mice

RNA from female gonads (C57/BL6xDBA/2 background) at different developmental stages (11.5dpc, 12.5dpc, 14.5dpc, 16.5dpc, 18.5dpc, 20.5dpc – dpc: days post coitum) together with RNA from adult testes and 17 different somatic tissues was used for transcriptome comparison. Overall design: For each developmental stage from female gonads RNA was extracted from at least a pool of 34 ovaries. Total RNA samples from different mouse somatic tissues were purchased via Ambion (liver, brain, thymus, heart, lung, spleen and kidney) and Zyagen (mammary gland, pancreas, placenta, salivary gland, skeletal muscle, skin, small intestine, spinal cord, tongue and uterus)