NGS amplicon sequencing of 16S rRNA genes, mcrA, [FeFe]-hydrogenase genes and [NiFe]-hydrogenase genes from a pH-neutral Finnish fen soil. Puukkosuo fen microbes

In this study, we looked at fermentation and methanogenesis in a pH-neutral fen soil. To support physiological data from in situ measurements and microcosm incubations, we sequenced 16S rRNA amplicons as well as amplicons of genes potentially involved in fermentation and methanogenesis ([FeFe]- and [NiFe]-hydrogenases and mcrA).