Thermoanaerobacterium saccharolyticum (T. sacch) has a robust pyruvate to ethanol metabolic pathway that we would like to transfer to Clostridium thermocellum for the purpose of generating an organism
A new approach for modulating gene expression, based on randomization of promoter (spacer) sequences, was developed. The method was applied to chromosomal genes in Lactococcus lactis and shown to gene
As improvements in DNA synthesis technology and assembly methods make combinatorial assembly of genetic constructs increasingly accessible, methods for representing genetic constructs likewise need to
The monomer units in the Escherichia coli and Staphylococcus aureus cell wall peptidoglycans differ in the nature of the third amino acid in the l-alanyl-γ-d-glutamyl-X-d-alanyl-d-alanine side chain,
Mycelia were harvested by filtration and centrifugation from cultures grown for 36 h on liquid MM containing 1% Avicel as the sole carbon source with 0 or 1% DMF supplementation. All of the mycelia of