Effect of Akirin2 knockdown on the mRNA profile of human HL60 cells

The Akirin (AKR) family are transcription cofactors without catalytic or DNA-binding capability with a conserved function in the regulation of multiple biological processes (BPs) throughout the metazoan. Human akirin2 (AKR2) is involved in various BPs in cell type and stimulus-dependent manner by acting as a link between NF-?B and chromatin remodeling complexes (remodelers) for transcriptional regulation. The regulome (transcription factors/cofactors-target genes interactions) plays an important role in the regulation of these BPs and the application of transcriptomics studies can be critical to better understanding the role of this transcription factors/cofactors as AKR2. In this study, we focused in AKR2 regulome to clarify its function as conserved regulatory cofactor. To assess this, RNA-seq analysis were conducted in wild type (WT) and AKR2-knock down (KO) human Caucasian promyelocytic leukemia HL60 cells. Results have shown a different gene expression profile that allowed us to identify immune response genes as putative AKR2 functional complements providing a better understanding of AKR2 regulome and its possible chromatin remodeling function. Overall design: Total mRNA profile in WT and AKR2 KO HL60 cells (three biological replicates).